Product overview Description Anti-PSMB7 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human PSMB7 Reactivity Human, Mouse, Rat Content 0.2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol PSMB7 Full name proteasome (prosome, macropain) subunit, beta type, 7 Synonyms Z Swissprot Q99436
Target Background The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.
Applications Immunohistochemistry Predicted cell location: Cytoplasm and Nucleus Positive control: Human colon cancer Recommended dilution: 25-100 Predicted cell location: Cytoplasm and Nucleus Positive control: Human gastric cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml221776(PSMB7 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml221776(PSMB7 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:30 kDa Positive control:293T and hepG2 cells, human testis and lung cancer tissue Recommended dilution: 500-2000
Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-4: 293T cells, hepG2 cells, human testis tissue, Human lung cancer tissue Primary antibody: ml221776(PSMB7 Antibody) at dilution 1/310 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds