Product overview Description Anti-COPE rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human COPE Reactivity Human, Mouse Content 0.2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol COPE Full name coatomer protein complex subunit epsilon Synonyms epsilon-COP Swissprot O14579
Target Background The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 20-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml224625(COPE Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:34 kDa Positive control:Hela,A375 and PC-3 cell lysates Recommended dilution: 200-1000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: Hela,A375 and PC-3 cell lysates Primary antibody: ml224625(COPE Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds