Product overview Description Anti-APOBEC3G rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human APOBEC3G Reactivity Human Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol APOBEC3G Full name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G Synonyms A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1 Swissprot Q9HC16
Target Background This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human thyroid cancer Recommended dilution: 25-100 Predicted cell location: Cytoplasm Positive control: Human gastric cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221663(APOBEC3G Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using ml221663(APOBEC3G Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:46 kDa Positive control:PC3, Jurkat and Raji cells Recommended dilution: 200-1000
Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-3: PC3 cells, Jurkat cells, Raji cells Primary antibody: ml221663(APOBEC3G Antibody) at dilution 1/240 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds