- 询价
- gelatins
- JLC221496
- 上海
- 2025年07月09日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-ADAM10 antibody
- 宿主：
Rabbit
- 浓度：
0.3 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-ADAM10 antibody
Cat. No. JLC221496
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ADAM10 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ADAM10
Reactivity Human, Mouse, Rat
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ADAM10
Full name ADAM metallopeptidase domain 10
Synonyms kuz; AD10; MADM; CD156c; HsT18717
Swissprot O14672
Target Background
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP).
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221496(ADAM10 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml221496(ADAM10 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:84 kDa
Positive control:PC3 cells
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: PC3 cells
Primary antibody: ml221496(ADAM10 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute
ELISA
Recommended dilution: 1000-2000
Cat. No. JLC221496
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ADAM10 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ADAM10
Reactivity Human, Mouse, Rat
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ADAM10
Full name ADAM metallopeptidase domain 10
Synonyms kuz; AD10; MADM; CD156c; HsT18717
Swissprot O14672
Target Background
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP).
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221496(ADAM10 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml221496(ADAM10 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:84 kDa
Positive control:PC3 cells
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: PC3 cells
Primary antibody: ml221496(ADAM10 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute
ELISA
Recommended dilution: 1000-2000
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Anti-ADAM10 antibody
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