Product overview Description Anti-UBA7 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human UBA7 Reactivity Human Content 0.5 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol UBA7 Full name ubiquitin-like modifier activating enzyme 7 Synonyms D8; UBE2; UBA1B; UBE1L Swissprot P41226
Target Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.
Applications Immunohistochemistry Predicted cell location: Nucleus and Cytoplasm Positive control: Human colorectal cancer Recommended dilution: 25-100 Predicted cell location: Nucleus and Cytoplasm Positive control: Human tonsil Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ml224149(UBA7 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml224149(UBA7 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:112 kDa Positive control:HEPG2 and 293T cell lysates Recommended dilution: 200-1000
Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-2: HEPG2 and 293T cell lysates Primary antibody: ml224149(UBA7 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds