- 询价
- gelatins
- JLC221318
- 上海
- 2025年07月14日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-F13A1 antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC221318
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-F13A1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human F13A1
Reactivity Human, Mouse
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol F13A1
Full name Coagulation factor XIII, A1 polypeptide
Synonyms F13A
Swissprot P00488
Target Background
This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot.
Applications
Immunohistochemistry
Predicted cell location: Secreted, Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 15-50 Predicted cell location: Secreted, Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 15-50
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml221318(F13A1 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml221318(F13A1 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:83 kDa
Positive control:Human fetal muscle tissue
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: Human fetal muscle tissue
Primary antibody: ml221318(F13A1 Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 30 minutes
ELISA
Recommended dilution: 1000-2000
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