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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-SRC antibody
- 宿主:
Rabbit
- 浓度:
0.2 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-SRC antibody
Cat. No. JLC221267
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-SRC rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human SRC
Reactivity Human, Mouse, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol SRC
Full name SRC proto-oncogene, non-receptor tyrosine kinase
Synonyms ASV; SRC1; c-SRC; p60-Src
Swissprot P12931
Target Background
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lymphoma
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human lymphoma tissue using ml221267(SRC Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml221267(SRC Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:60 kDa
Positive control:PC3 cells
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane: PC3 cells
Primary antibody: ml221267(SRC Antibody) at dilution 1/200
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 1000-2000
Cat. No. JLC221267
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-SRC rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human SRC
Reactivity Human, Mouse, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol SRC
Full name SRC proto-oncogene, non-receptor tyrosine kinase
Synonyms ASV; SRC1; c-SRC; p60-Src
Swissprot P12931
Target Background
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lymphoma
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human lymphoma tissue using ml221267(SRC Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml221267(SRC Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:60 kDa
Positive control:PC3 cells
Recommended dilution: 200-1000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane: PC3 cells
Primary antibody: ml221267(SRC Antibody) at dilution 1/200
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 2 minutes
ELISA
Recommended dilution: 1000-2000
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Anti-SRC antibody
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