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- 西班牙/IMMUNOSTEP
- 西班牙
- PI/RNASE
- 2025年07月29日
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文献和实验A general protocol for staining cell for cytometry analysis
(Cat. No. 66121A) : 0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1X prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1X PBS buffer. Store at RT. Recommended for use in parallel with Annexin
a highly fluorescent adduct that can be excited at 488 nm with a broad emission centred around 600 nm. Since PI can also bind to double-stranded RNA, it is necessary to treat the cells with RNase for optimal DNA resolution. The excitation of PI at 488 nm
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
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