Product overview Description Anti-MCM7 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human MCM7 Reactivity Human, Mouse Content 0.2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol MCM7 Full name minichromosome maintenance complex component 7 Synonyms MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3 Swissprot P33993
Target Background The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Applications Immunohistochemistry Predicted cell location: Cytoplasm, Nucleus Positive control: Human ovarian cancer Recommended dilution: 15-50 Predicted cell location: Cytoplasm, Nucleus Positive control: Human colon cancer Recommended dilution: 15-50
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml220965(MCM7 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml220965(MCM7 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:81 kDa Positive control:Jurkat cells Recommended dilution: 500-2000
Gel: 10%SDS-PAGE Lysate: 50 μg Lane: Jurkat cells Primary antibody: ml220965(MCM7 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds