Product overview Description Anti-IRF6 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human IRF6 Reactivity Human, Mouse Content 0.4 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol IRF6 Full name interferon regulatory factor 6 Synonyms LPS; PIT; PPS; VWS; OFC6; PPS1; VWS1 Swissprot O14896
Target Background This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.
Applications Immunohistochemistry Predicted cell location: Nucleus and Cytoplasm Positive control: Human cervical cancer Recommended dilution: 50-200 Predicted cell location: Nucleus and Cytoplasm Positive control: Human lung cancer Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml220876(IRF6 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml220876(IRF6 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:53 kDa Positive control:231 cells, mouse kidney and lung tissue Recommended dilution: 200-1000 Gel: 8%SDS-PAGE
Lysate: 40 μg Lane 1-3: 231 cells, mouse kidney tissue, Mouse lung tissue Primary antibody: ml220876(IRF6 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 minutes