Product overview Description Anti-IMMT rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human IMMT Reactivity Human, Mouse Content 0.3 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol IMMT Full name inner membrane protein, mitochondrial Synonyms HMP, P87, P89, PIG4, PIG52, MINOS2, P87/89 Swissprot Q16891
Target Background Mitochondrial inner membrane protein is a protein that in humans is encoded by the IMMT gene.IMMT has been shown to interact with BAT2.The mitochondrial inner membrane (IM) serves as the site for ATP production by hosting the oxidative phosphorylation complex machinery most notably on the crista membranes. Disruption of the crista structure has been implicated in a variety of cardiovascular and neurodegenerative diseases.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human colon cancer Recommended dilution: 15-50 Predicted cell location: Cytoplasm Positive control: Human breast cancer Recommended dilution: 15-50
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml220856(IMMT Antibody) at dilution 1/12, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml220856(IMMT Antibody) at dilution 1/12, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:84 kDa Positive control:Hela and 231 cells Recommended dilution: 500-2000 Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Hela cells, 231 cells Primary antibody: ml220856(IMMT Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute