Product overview Description Anti-RNF214 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human RNF214 Reactivity Human, Mouse Content 0.2 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol RNF214 Full name ring finger protein 214 Synonyms Swissprot Q8ND24
Target Background RNF214 (RING finger protein 214) is a 703 amino acid protein that contains one RING-type zinc finger and is encoded by a gene that maps to human chromosome 11q23.3. Chromosome 11 houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 10-50
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223047(RNF214 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:78 kDa Positive control:823, PC3, 231 and hepg2 cells Recommended dilution: 500-2000
Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-4: 823 cells, PC3 cells, 231 cells, hepg2 cells Primary antibody: ml223047(RNF214 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds