- $875
- LSBio
- To be determined
- LS-F13928
- 美国
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F13928 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat GAA / Alpha-Glucosidase, Acid. It is based upon a Custom assay principle.Specifications
TypeCustom ELISA kit
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Target
GAA / Alpha-Glucosidase, Acid
Reactivity
Rat
Manual
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
To be determined
Sensitivity
To be determined
Storage
Short term: 4°C; Long term: see manual.
Kit Components
Typical components include:
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Wash Buffer (25x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About GAA / Alpha-Glucosidase, Acid
P10253 NM_000152 NP_000143.2
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文献和实验, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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