Product overview Description Anti-FAR2 rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Fusion protein of human FAR2 Reactivity Human, Mouse Content 0.6 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol FAR2 Full name fatty acyl CoA reductase 2 Synonyms MLSTD1; SDR10E2 Swissprot Q96K12
Target Background This gene belongs to the short chain dehydrogenase/reductase superfamily. It encodes a reductase enzyme involved in the first step of wax biosynthesis wherein fatty acids are converted to fatty alcohols. The encoded peroxisomal protein utilizes saturated fatty acids of 16 or 18 carbons as preferred substrates. Alternatively spliced transcript variants have been observed for this gene. Related pseudogenes have been identified on chromosomes 2, 14 and 22.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human thyroid cancer Recommended dilution: 50-200 Predicted cell location: Cytoplasm Positive control: Human liver cancer Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml222347(FAR2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml222347(FAR2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Western blotting Predicted band size:59 kDa Positive control:Human fetal liver tissue Recommended dilution: 1000-5000
Gel: 6%SDS-PAGE Lysate: 40 μg Lane: Human fetal liver tissue Primary antibody: ml222347(FAR2 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds