Product overview Description Anti-CATSPER3 rabbit polyclonal antibody Applications ELISA, WB Immunogen Fusion protein of human CATSPER3 Reactivity Human, Mouse Content 0.4 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol CATSPER3 Full name cation channel, sperm associated 3 Synonyms CACRC Swissprot Q86XQ3
Target Background CatSpers (cation channel, sperm associated proteins) are ion transport proteins located on the surface of sperm cells in the principal piece of the sperm tail. CatSpers are vital to sperm motility, fertilization and cAMP-mediated calcium influx in sperm. There are four CatSper proteins in mammalian sperm, namely CatSper (or CatSper1), CatSper2, CatSper3 and CatSper4. CatSper proteins contain a single, six-transmembrane-spanning segment and exhibit the voltage-dependent Ca2+ channel four-repeat structure. CatSper proteins are believed to assemble into a heterotetrameric complex, forming an alkalinization-activated Ca2+-selective channel. Mutations in any of the genes encoding CatSper family proteins can result in male infertility. CatSper3 plays an important role in the hyperactivated motility of sperm cells, a process that is required in the preparation of sperm for fertilization.
Applications Western blotting Predicted band size:46 kDa Positive control:Mouse testis tissue Recommended dilution: 500-2000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Mouse testis tissue Primary antibody: ml222040(CATSPER3 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes