LS-F7980 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human FZD6 / Frizzled 6 in samples of Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of FZD6 / Frizzled 6 as low as 0.119 nanograms per millilter.
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Frizzled Homolog 6 (FZD6) No significant cross-reactivity or interference between Frizzled Homolog 6 (FZD6) and analogs was observed.
Manual
Intended Sample Types
Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.313 - 20 ng/ml
Sensitivity
0.119 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. O60353NM_003506NP_003497.2