- ￥6800
- GeneTex
- 美国
- GTX57213
- 2025年07月12日
- WB, ICC/IF, FACS, Functional Assay
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human iPS cell line, Tic, derived from human fetus lung cells.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX57213
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS, Functional Assay
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
lacto-N-fucopentaose I
- 抗体英文名：
lacto-N-fucopentaose I antibody [R-17F]
- 抗体名：
lacto-N-fucopentaose I 抗体 [R-17F]
- 规格：
100 μl
Cultured human iPS cells were stained with R-17F, SSEA-3, and SSEA-4 antibodies. [bars: 100μm]
R-17F stained the entire surface of the cell membranes equally, while the staining by SSEA-3 and SSEA-4 antibodies are not evenly. This suggests that R-17F epitope is expressed ubiquitously all over the human iPS cells.
WB analysis of human iPS and HEK293 cell lysate using GTX57213 lacto-N-fucopentaose I antibody [R-17F].
One major positive band and several minor bands were specific to human iPS cells, and any positive band was not obtained with HEK293 cells.
Human iPS cell: LNFP I positive
HEK293 cell lysate: Negative Control
Loading : 5ug per lane
Dilution : 1:2000
R-17F is reported to exhibit potent dose-dependent cytotoxicity when it is added to living human iPS/ES cells.
[Left] After the incubation of iPS cell suspension with R-17F at 4ºC for only 45 minutes, the percentage of viable cells decreased concentration-dependently (red triangles). Blue squares : effects of the isotype (IgG1)-matching control antibody (anti-α-MBP) as Negative Control.
[Right] When R-17F-treated iPS cells were incubated with a small amount (0.025-0.1 μg) of the secondary antibody (goat anti-mouse IgG1 antibody), the cytotoxic effect of R-17F was enhanced significantly in a dose-dependent manner (red triangles).
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人sTRAIL R ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和裂解物及唾液其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sTRAIL R 单抗包被于酶标板上，标准品和样品中的 sTRAIL R与单抗结合，加入生物
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
., Finstad, J., Williams, Jr., R. C. 1970. Phylogenetic insight into evolution of mammalian Fc fragment of g G globulin using staphylococcal protein A. J. Immunol. 104:140-147. Kronvall, G. 1973. A surface component in group A, C, and G streptococci
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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