LS-F33911 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse FERMT1 / Kindlin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of FERMT1 / Kindlin as low as 46.9 picograms per milliliter.
More FERMT1 / Kindlin Products Top of Page
FERMT1, C20orf42, DTGCU2, Fermitin family homolog 1, Kindlin syndrome protein, KIND1, Kindlin 1, UNC112A, URP1, UNC112 related protein 1, Fermitin family member 1, Kindlerin, Kindlin, Kindlin-1, Unc-112-related protein 1
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
78 - 5000 pg/ml
Sensitivity
46.9 pg/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fermt1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fermt1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fermt1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofFermt1 can be calculated.
Restrictions
Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites. May mediate TGF-beta 1 signaling in tumor progression.Q9BQL6NM_017671NP_060141.3