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Cyclooxygenase 2 Rabbit Polycl

onal Antibody抗体,orb501007,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb501007
  • 英国
  • 2025年12月03日
  • ICC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 技术资料
    • 抗体名

      Cyclooxygenase 2 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Cyclooxygenase 2 Rabbit Polyclonal Antibody

    • 靶点

      Ptgs2

    • 浓度

      1mg/ml

    • 应用范围

      ICC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb501007

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 (501-604/604aa)

    • 规格

      50 ul

    产品描述:Cyclooxygenase 2 Rabbit Polyclonal Antibody

    别名:PTGS2; COX 2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; COX2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN.

    免疫原:KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 (501-604/604aa)

    预测反应性:Bovine, Canine, Porcine, Rabbit, Sheep

    分子量:65 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P35354

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): HepG2 (blue). Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2/FITC Conjugated antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 2% Fixative (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Blank control: U937. Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2 antibody (orb501007), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片3
    Blank control: U937. Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2 antibody (orb501007), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片4
    Blank control: U937. Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2 antibody (orb501007), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片5
    Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclooxygenase 2) Polyclonal Antibody, Unconjugated (orb501007) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片6
    Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclooxygenase 2) Polyclonal Antibody, Unconjugated (orb501007) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片7
    Sample: Lane 1: Recombinant human COX2 protein, Primary: Anti-Cyclooxygenase 2 (orb501007) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 40 kD.

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