活性定义:One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a
polynucleotide fraction (adsorbed on DE-81) in 30 min at 70℃. Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25℃), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/ml BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/ml [3H]dTTP。
纯度:不含DNA内切酶、外切酶和磷酸酯酶,不含RNA酶,满足常规PCR反应要求。
酶储存溶液:20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycerol。
10×PCR Buffer (with Mg2+):100 mM Tris-HCl (pH 8.8 at 25℃), 500 mM KCl, 15mM MgCl2, 0.8% (v/v) Nonidet P40。