Product overview
Description Anti-RDX rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human RDX
Reactivity Human, Mouse
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol RDX
Full name radixin
Synonyms DFNB24
Swissprot P35241
Target Background
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human tonsil
Recommended dilution: 100-300 Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 100-300
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml220615(RDX Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml220615(RDX Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:69 kDa
Positive control:Hela cells and human brain tissue, 293T cells, human hepatocellsular carcinoma tissue and NIH/3T3 cells
Recommended dilution: 500-2000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-5: Hela cells, human brain tissue, 293T cells, human hepatocellular carcinoma tissue, NIH/3T3 cells
Primary antibody: ml220615(RDX Antibody) at dilution 1/600
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 seconds