Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
适应物种:
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
免疫原:
详见说明书
亚型:
IGg
形态:
粉末、液体、冻干粉
应用范围:
IF=1:50-200
浓度:
1mg/ml
保存条件:
-20 °C
规格:
100ul
FITC标记的磷酸化自噬相关蛋白3抗体
英文名称
Anti-phospho-ATG3 (Tyr18)/FITC
中文名称
FITC标记的磷酸化自噬相关蛋白3抗体
别 名
2610016C12Rik; Apg 3; APG3; APG3 autophagy 3 like; APG3 like; APG3-like; APG3L; Apg3p; ATG 3; ATG3; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3_HUMAN; Autophagy Apg3p/Aut1p like; Autophagy related protein 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; OTTHUMP00000214547; OTTHUMP00000214548; PC3 96; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3.
规格价格
100ul/2980元 购买 大包装/询价
说 明 书
100ul
产品类型
磷酸化抗体
研究领域
肿瘤 细胞生物 神经生物学 信号转导 线粒体 泛素
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用
IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
36kDa
性 状
Lyophilized or Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated Synthesised phosphopeptide derived from human ATG3 around the phosphorylation site of Tyr18
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍
background: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Function: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Subunit: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L.
Subcellular Location: Cytoplasm.
Tissue Specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Post-translational modifications: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy (By similarity).
Similarity: Belongs to the ATG3 family.
Database links:
Entrez Gene: 64422 Human
Entrez Gene: 67841 Mouse
Entrez Gene: 171415 Rat
Omim: 609606 Human
SwissProt: Q9NT62 Human
SwissProt: Q9CPX6 Mouse
SwissProt: Q6AZ50 Rat
Unigene: 477126 Human
Unigene: 41775 Mouse
Unigene: 3084 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.