LS-F54686 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human EZH2 in samples of Cell Lysates, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of EZH2 as low as 7.81 picograms per milliliter.
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EZH2, ENX1, Enhancer of zeste homolog 2, KMT6, KMT6A, Lysine N-methyltransferase 6, WVS2, Enhancer of zeste, ENX-1
Reactivity
Human
Manual
Intended Sample Types
Cell Lysates, Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
31.25 - 2000 pg/ml
Sensitivity
7.81 pg/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Store at 2°C to 8°C. Do not use after expiration date. Maybe be stored for up to 1 month after opeing.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Q15910NM_004456NP_004447.2