- $615
- LSBio
- 详见官方网站
- LS-F1979
- 美国
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human, Rat
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
Performance 100% Guaranteed
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Product Description
LS-F1979 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human ETS1 / ETS-1 in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.More ETS1 / ETS-1 Products
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Specifications
TypeCell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
ETS1 / ETS-1
Synonyms
ETS1, Ets protein, ETS-1, EWSR2, p54, Protein C-ets-1
Reactivity
Mouse, Human, Rat
Specificity
ETS1
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Cell Culture Microplate
- Blocking Buffer
- Detection Antibodies
- HRP-Conjugate
- Substrate
- Stop Solution
- Crystal Violet Solution
- SDS Solution
- Adhesive Plate Sealers
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About ETS1 / ETS-1
C-ets-1 is a nuclear ETS proto-oncogene product that activate transcription by interaction with purine-rich sequences present in the promoter/enhancer region of the T-Cell antigen receptor (TCRA) gene. In many cancers such as leukemia and lymphomas, the ETS-1 gene is either translocated or rearranged and amplified. In contrast, ectopic expression of ETS-1 in colon cancer cell lines that do not normally express the gene, suppresses tumorigenicity at a transcriptional level. P14921 NM_005238 NP_005229.1
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Cell-Based ELISA Platform Overview
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文献和实验, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
antiplatelet autoantibodies from autoimmune thrombocytopenic purpura-prone (NZW x BXSB)F1 mice. Blood 82(3): 837-44. ELISA相关仪器试剂设备 酶标仪 洗板机 化学发光检测仪 ELISA检测试剂盒
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