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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
92.44 - 5333 pg/ml
- 供应商:
康朗生物
- 规格:
96T
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Product Description
LS-F3333 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human EPOR / EPO Receptor in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of EPOR / EPO Receptor as low as 92.44 picograms per milliliter.More EPOR / EPO Receptor Products
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
EPOR / EPO Receptor
Synonyms
EPOR, EPO Receptor, EPO-R, Erythropoietin receptor
Reactivity
Human
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
2 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
92.44 - 5333 pg/ml
Sensitivity
92.44 pg/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About EPOR / EPO Receptor
EPOR / EPO Receptor is the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. P19235 NM_000121 NP_000112.1
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Sandwich ELISA Platform Overview
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
phagocytosis. J. Cell Biol. 153:1369‐1380. Masilamani, M., Peruzzi, G., Borrego, F., and Coligan, J.E. 2009. Endocytosis and intracellular trafficking of human natural killer cell
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