Human EPO / Erythropoietin ELI

Human EPO / Erythropoietin ELISA Kit (Sandwich ELISA) - LS-F24490

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LS-F24490-1
1 plate
$1,340 USD
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Product Description

LS-F24490 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human EPO / Erythropoietin in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of EPO / Erythropoietin as low as 0.4 milli international units per milliliter.
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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
EPO / Erythropoietin
Synonyms
 
EPO, Epoetin, EP, MVCD2, Erythropoietin
Reactivity
 
Human
Specificity
 
For the detection and quantification of endogenous levels of natural and/or recombinant Human EPO proteins.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
3.13 - 200 mIU/ml
Sensitivity
 
0.4 mIU/ml
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• User Manual
• 96-well Microplate
• Detection Reagents
• Wash Buffer
• Substrate
• Stop Solution
• Adhesive Plate Sealers

Background
 
Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About EPO / Erythropoietin
 
is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. Uniprot: P01588 NCBI: NM_000799NP_000790.2

 

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