LS-F7554 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse EPHB3 / EPH Receptor B3 in samples of Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of EPHB3 / EPH Receptor B3 as low as 0.051 nanograms per millilter.
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Ephrin Type B Receptor 3 (EPHB3) No significant cross-reactivity or interference between Ephrin Type B Receptor 3 (EPHB3) and analogs was observed.
Manual
Intended Sample Types
Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.051 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. P54753NM_004443NP_004434.2