LS-F1494 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human EIF2S1 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle.
More EIF2S1 Products Top of Page
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
EIF2 is a translation initiation factor that catalyzes the first regulated step of protein synthesis, promoting the binding of the initiator tRNA to 40s ribosomal subunits. eIF2 is composed of 3 non identical subunits, alpha, beta and gamma. Binding occurs as a ternary complex of methionyl-tRNA, eIF2 and GTP. The rate of complex formation is modulated by the phosphorylation state of eIF2alpha. Phosphorylation of eIF2alpha has been shown to occur in response to heat shock and hypoxic stress. P05198NM_004094NP_004085.1