LS-F5387 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rabbit EDN1 / Endothelin 1 in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of EDN1 / Endothelin 1 as low as 2.74 picograms per milliliter.
More EDN1 / Endothelin 1 Products Top of Page
Endothelin 1 (EDN1) No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
6.17 - 500 pg/ml
Sensitivity
2.74 pg/ml
Assay Time
2.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
EDN1 / Endothelin 1 is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.P05305NM_001955NP_001946.3