LS-F51815 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human EBI3 / IL-27B in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of EBI3 / IL-27B as low as 3.9 picograms per milliliter.
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This assay has high sensitivity and excellent specificity for detection of human EBI3. No significant cross-reactivity or interference between human EBI3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human EBI3 and all the analogues, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.6 - 1000 pg/ml
Sensitivity
3.9 pg/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<8% / Inter-Assay: CV<10%
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Q14213NM_005755NP_005746.2