After reconstitution freeze in small aliquots (e.g. 1 ul) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
克隆性:
多克隆
适应物种:
Rabbit
供应商:
上海联迈生物工程有限公司
靶点:
Ca2+-ATPase 3 (human plasma membrane)
规格:
200 µl
商品分类:Antibodies against Ca2+ exchang
研究领域:Ca2+ - pumps
靶点/蛋白质:Ca2+-ATPase 3 (human plasma membrane)
来源:Rabbit
存储溶液:Lyophilized whole serum (no preservatives).
参考文献:1. Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma-membrane Ca2+-pump. A study using specific antibodies. J. Biol. Chem. 19: 12184- 12190. 2. Stauffer T.P., Guerini D., Celio M.R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+-pump isoforms in the rat brain. Brain Res. 748:21-29.
应用:Immunohistochemistry : 1: 1'000 - 1: 2'000. Immunoblots: 1: 1'000 - 1: 2'000. For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.
官网链接:https://www.swant.com/pdfs/Rabbit_anti_Ca-Pump_3.pdf
商品关键词:Antiserum,PMCA3,Swant
简单描述:The antiserum against Ca2+-ATPase, isoform 3, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labelling of cryostatesections of 4% paraformaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots of brain membranes or primary nerve cell cultures. It can also be used for immunoprecipitation. The serum was inactivated at 50°C for 30 minutes.
Product: Antiserum
Ref: 10
Code No: PMCA3
Lot No: #72
Reconstitution: with 200 ul of bidistilled water.