After reconstitution freeze in small aliquots (e.g. 1 ul) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
克隆性:
多克隆
适应物种:
Mouse monoclonal, IgG1
供应商:
上海联迈生物工程有限公司
靶点:
Na+-Ca2+ exchanger protein (cardiac)
规格:
200 µl
商品分类:Antibodies against Ca2+ exchang
研究领域:Ca2+ - exchangers
靶点/蛋白质:Na+-Ca2+ exchanger protein (cardiac)
来源:Mouse monoclonal, IgG1
存储溶液:Lyophilized, concentrated supernatant.
参考文献:Porzig H., Li Z., Nicoll D.A., Philipson K.D. (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies. Am. J. Physiol. 265:C78 -C756, 1993
应用:Immunohistochemistry: 1: 500; 1: 1’000 Immunoblots and immunoprecipitation: 1: 500; 1: 1'000 (use milk for blocking). A strong 70 kDa band is found in addition to the 116 kDa of the integral exchanger. Often the HMW band is a double band 116/ 112 kDa band. The titer was determined using the indirect-peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.
官网链接:https://www.swant.com/pdfs/Monoclonal_anti_Na-Ca-exchanger.pdf
商品关键词:Conc. Supernat,R3F1,Swant
简单描述:The monoclonal antibody against the canine Na+ -Ca2+-exchanger, recognizes two neighboring but non-overlapping sequences of the hydrophilic region of the exchanger, connecting the putative transmembrane segments 5 and 6. The antibody was evaluated for specificity and potency by immunoblotting, and immunoprecipitation (see reference). It recognizes virtually only NCX1 in monkey, horse, dog, rat, bovine and mouse tissue. At higher concentrations it reacts faintly with NCX2, whereas NCX3 is not recognized. Both splice variants with exon A (heart, nervous system) and exon B (kidney, glia) are recognized.
Product: Conc.
SupernatRef: 9
Code No: R3F1
Lot No: #mr05
Reconstitution: with 200 ul of bidistilled water.
IgG Subtype: IgG1