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- 详细信息
- 技术资料
- 抗体名:
磷酸化SH2结构域转化蛋白1抗体图片
- 抗体英文名:
Anti-phospho-SHC(Tyr239/240)
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海博湖
- 库存:
大量
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated Synthesised phosphopeptide derived from human SHC around the phosphorylation site of Tyr239/240
- 规格:
0.2ml/200μg
磷酸化SH2结构域转化蛋白1抗体图片Anti-SPHK1/FITC 荧光素标记鞘醇激酶1抗体IgGMulti-class antibodies规格: 0.2ml
TIMP-1(Tissue Inhibitor of Metalloproteinase-1) 金属蛋白酶组织抑制因子-1(抗原)Multi-class antibodies规格: 0.5mg
关节炎相关基因抗体 Anti-PADI4/PAD4 0.1ml
SAP62 英文名称: 剪接体相关蛋白62抗体 0.2ml
EIF2C 英文名称: 真核翻译起始因子2C1抗体 0.2ml
Rhesus antibody Rh Phospho-NFKB1(Ser893) 化细胞核因子p50/k基因结合核因子抗体 规格 0.1ml
TIMP-1(Tissue Inhibitor of Metalloproteinase-1) 金属蛋白酶组织抑制因子-1(抗原)Multi-class antibodies规格: 0.5mg
英文名称 Anti-phospho-SHC(Tyr239/240)
中文名称 磷酸化SH2结构域转化蛋白1抗体图片
别 名 p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming protein; SHC1; SHCA; Src homology 2 domain containing transforming protein C1.
磷酸化SH2结构域转化蛋白1抗体图片浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 信号转导 转录调节因子
蛋白分子量 predicted molecular weight: 41kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human SHC around the phosphorylation site of Tyr239/240
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化SH2结构域转化蛋白1抗体图片产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
磷酸化SH2结构域转化蛋白1抗体图片抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,Elisa,WB,免疫荧光等实验。
FoxP1(Forkhead box P1) FoxP1(T细胞转录因子)抗原Multi-class antibodies规格: 0.5mg
CCNA1 Cyclin A1 / CCNA1 鼠单抗 (PE) Human
Rhesus antibody Rh phospho-Dnmt1(Ser154) 化DNA甲基转移酶1抗体 规格 0.1ml
杂交袋(15×25cm) 100张/包 国产
ZFYVE27 英文名称: 指FYVE结构域蛋白27抗体 0.2ml
DIAPH2 英文名称: DIAPH2蛋白抗体 0.2ml
CCNA1 Cyclin A1 / CCNA1 鼠单抗 (PE) Human
ZNF434 英文名称: 抑癌蛋白5/指蛋白434抗体 0.2ml
Defensin alpha 5 英文名称: 防御素α5抗体 0.2ml
整合素样金属蛋白酶与凝血酶1型-7(抗体) Anti-ADAM-TS7 0.1ml
Anti-CD86/B7-2/RBITC 红色罗丹明标记CD86蛋白抗体抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-FAS (Tyr291) 化载脂蛋白A1抗体 规格 0.1ml
CRF (Corticotropin-Releasing Factor) 促肾上腺皮质激素释放因子Multi-class antibodies规格: 0.5mg
The regeneration (REG) family consists of secretory proteins involved in liver, pancreatic, gastric and intestinal cell proliferation or differentiation. Members of the REG family are divided into four subclasses, designated types I, II, III and IV. They have been implicated in the regulation of cell growth, tumorigenesis and the progression of cancer. Specifically, increased expression of Reg III Alpha results in pancreatic inflammation and liver carcinogenesis. Reg III Alpha, also de-signated pancreatitis-associated protein, localizes to the apical region of pancreatic acinar cells and is similar to the C-type lectin superfamily. The Reg III Alpha protein is mainly expressed in the intestine, with lower expression detected in healthy pancreas. It is also detected in pancreatic juice as a result of pancreatic inflammation.
Function : Might be a stress protein involved in the control of bacterial proliferation.
Subcellular Location : Secreted. Found in the apical region of pancreatic acinar cells.
Tissue Specificity : Constitutively expressed in the small intestine, moderately in colon and at an extremely low level in healthy pancreas.
DISEASE : Note=Overexpressed during the acute phase of pancreatitis.
Similarity : Contains 1 C-type lectin domainAnti-T7 tag/FITC 荧光素标记T7 tag标签抗体IgGMulti-class antibodies规格: 0.2ml
Nestin 巢蛋白(神经上皮干细胞蛋白)(抗原)Multi-class antibodies规格: 0.5mg
Smad 2/3抗体 Anti-Smad2/3 0.1ml
SMC5 英文名称: 染色体结构维持蛋白5抗体 0.2ml
ERK4 英文名称: 细胞外信号调节激酶4抗体 0.1ml
Rhesus antibody Rh phospho-MARK2(Thr595) 化丝酸/苏酸蛋白激酶MARK2抗体 规格 0.1ml
Nestin 巢蛋白(神经上皮干细胞蛋白)(抗原)Multi-class antibodies规格: 0.5mg
磷酸化SH2结构域转化蛋白1抗体图片SCF (Stem Cell Factor) 干细胞生长因子抗原Multi-class antibodies规格: 0.5mg
Anti-BCHE (NT) 丁酰胆碱酯酶抗体(N端)Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh PHD1/prolyl hydroxylase 脯酰羟化酶抗体 规格 0.2ml
Hepatitis C Virus 浓缩液 0.1ml 进口分装
UBE2 英文名称: 泛素激活酶2(泛素激活酶E1相关蛋白抗体) 0.2ml
CD3E 英文名称: CD3抗体 0.1ml
Anti-BCHE (NT) 丁酰胆碱酯酶抗体(N端)Multi-class antibodies规格: 0.1ml
磷酸化SH2结构域转化蛋白1抗体图片实验原理 :
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。
TIMP-1(Tissue Inhibitor of Metalloproteinase-1) 金属蛋白酶组织抑制因子-1(抗原)Multi-class antibodies规格: 0.5mg
关节炎相关基因抗体 Anti-PADI4/PAD4 0.1ml
SAP62 英文名称: 剪接体相关蛋白62抗体 0.2ml
EIF2C 英文名称: 真核翻译起始因子2C1抗体 0.2ml
Rhesus antibody Rh Phospho-NFKB1(Ser893) 化细胞核因子p50/k基因结合核因子抗体 规格 0.1ml
TIMP-1(Tissue Inhibitor of Metalloproteinase-1) 金属蛋白酶组织抑制因子-1(抗原)Multi-class antibodies规格: 0.5mg
英文名称 Anti-phospho-SHC(Tyr239/240)
中文名称 磷酸化SH2结构域转化蛋白1抗体图片
别 名 p46; p52; p52 SHC; p52SHC; p66; p66 SHC; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC 1; SHC A; Shc; SHC transforming protein 1; SHC transforming protein; SHC1; SHCA; Src homology 2 domain containing transforming protein C1.
磷酸化SH2结构域转化蛋白1抗体图片浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 信号转导 转录调节因子
蛋白分子量 predicted molecular weight: 41kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human SHC around the phosphorylation site of Tyr239/240
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化SH2结构域转化蛋白1抗体图片产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
磷酸化SH2结构域转化蛋白1抗体图片抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,Elisa,WB,免疫荧光等实验。
FoxP1(Forkhead box P1) FoxP1(T细胞转录因子)抗原Multi-class antibodies规格: 0.5mg
CCNA1 Cyclin A1 / CCNA1 鼠单抗 (PE) Human
Rhesus antibody Rh phospho-Dnmt1(Ser154) 化DNA甲基转移酶1抗体 规格 0.1ml
杂交袋(15×25cm) 100张/包 国产
ZFYVE27 英文名称: 指FYVE结构域蛋白27抗体 0.2ml
DIAPH2 英文名称: DIAPH2蛋白抗体 0.2ml
CCNA1 Cyclin A1 / CCNA1 鼠单抗 (PE) Human
ZNF434 英文名称: 抑癌蛋白5/指蛋白434抗体 0.2ml
Defensin alpha 5 英文名称: 防御素α5抗体 0.2ml
整合素样金属蛋白酶与凝血酶1型-7(抗体) Anti-ADAM-TS7 0.1ml
Anti-CD86/B7-2/RBITC 红色罗丹明标记CD86蛋白抗体抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-FAS (Tyr291) 化载脂蛋白A1抗体 规格 0.1ml
CRF (Corticotropin-Releasing Factor) 促肾上腺皮质激素释放因子Multi-class antibodies规格: 0.5mg
The regeneration (REG) family consists of secretory proteins involved in liver, pancreatic, gastric and intestinal cell proliferation or differentiation. Members of the REG family are divided into four subclasses, designated types I, II, III and IV. They have been implicated in the regulation of cell growth, tumorigenesis and the progression of cancer. Specifically, increased expression of Reg III Alpha results in pancreatic inflammation and liver carcinogenesis. Reg III Alpha, also de-signated pancreatitis-associated protein, localizes to the apical region of pancreatic acinar cells and is similar to the C-type lectin superfamily. The Reg III Alpha protein is mainly expressed in the intestine, with lower expression detected in healthy pancreas. It is also detected in pancreatic juice as a result of pancreatic inflammation.
Function : Might be a stress protein involved in the control of bacterial proliferation.
Subcellular Location : Secreted. Found in the apical region of pancreatic acinar cells.
Tissue Specificity : Constitutively expressed in the small intestine, moderately in colon and at an extremely low level in healthy pancreas.
DISEASE : Note=Overexpressed during the acute phase of pancreatitis.
Similarity : Contains 1 C-type lectin domainAnti-T7 tag/FITC 荧光素标记T7 tag标签抗体IgGMulti-class antibodies规格: 0.2ml
Nestin 巢蛋白(神经上皮干细胞蛋白)(抗原)Multi-class antibodies规格: 0.5mg
Smad 2/3抗体 Anti-Smad2/3 0.1ml
SMC5 英文名称: 染色体结构维持蛋白5抗体 0.2ml
ERK4 英文名称: 细胞外信号调节激酶4抗体 0.1ml
Rhesus antibody Rh phospho-MARK2(Thr595) 化丝酸/苏酸蛋白激酶MARK2抗体 规格 0.1ml
Nestin 巢蛋白(神经上皮干细胞蛋白)(抗原)Multi-class antibodies规格: 0.5mg
磷酸化SH2结构域转化蛋白1抗体图片SCF (Stem Cell Factor) 干细胞生长因子抗原Multi-class antibodies规格: 0.5mg
Anti-BCHE (NT) 丁酰胆碱酯酶抗体(N端)Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh PHD1/prolyl hydroxylase 脯酰羟化酶抗体 规格 0.2ml
Hepatitis C Virus 浓缩液 0.1ml 进口分装
UBE2 英文名称: 泛素激活酶2(泛素激活酶E1相关蛋白抗体) 0.2ml
CD3E 英文名称: CD3抗体 0.1ml
Anti-BCHE (NT) 丁酰胆碱酯酶抗体(N端)Multi-class antibodies规格: 0.1ml
磷酸化SH2结构域转化蛋白1抗体图片实验原理 :
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。
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磷酸化SH2结构域转化蛋白1抗体图片
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