- ￥277
- Genwaybio
- 美国
- GWB-760365
- 2025年06月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 抗体英文名：
HBsAg adw2 subtype Q129L mutant form recombinant
- 规格：
100ug，200ug，1mg
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文献和实验Identification of a Mutant Kinase/ATP Analog Pair
, it is necessary to screen ATP analogs for their compatibility with the kinase mutant. This step requires an active form of the wild-type and mutant kinase, either as recombinant activated protein, or as protein that has been immunoprecipitated from transfected
同位素法测定底物磷酸化活性方法 Phosphorylation of Substrates
identification of kinase-associated substrates by immunoprecipitating a tagged form of the mutant kinase from transfected COS-1 cells and performing a kinase reaction by the addition of [γ-32 P]ATP analog. The second approach involves the addition of recombinant
4. Add purified, active recombinant mutant kinase to 100 µg of cell lysate. We use 10-100 ng of activated ERK2, but this must be determined empirically according to the specific activity of the kinase of interest.
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