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- 详细信息
- 技术资料
- 抗体名:
14-3-3 protein ζ/δ抗体图片
- 抗体英文名:
Anti-14-3-3 zeta/delta
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海博湖
- 库存:
大量
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta
- 规格:
0.2ml/200μg
英文名称 Anti-
中文名称 14-3-3 protein ζ/δ抗体图片
别 名 14 3 3 protein delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2;
14-3-3 protein ζ/δ抗体图片浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
产品类型 一抗
研究领域 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 细胞类型标志物
蛋白分子量 predicted molecular weight: 27kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
14-3-3 protein ζ/δ抗体图片产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subunit : Interacts with CDK16 and BSPRY. Interacts with WEE1 (C-terminal). Interacts with SAMSN1. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with BCL2L11. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2.
Subcellular Location : Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
Post-translational modifications : The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.
Similarity : Belongs to the
Database links : UniProtKB/Swiss-Prot: P63104.1
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,Elisa,WB,免疫荧光等实验。
14-3-3 protein ζ/δ抗体图片实验原理 :
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。
Anti-TTF-1/FITC 荧光素标记甲状腺核转录因子-1/甲状腺特异增强子结合蛋白抗体IgGMulti-class antibodies规格: 0.2ml
Trk B 神经生长因子受体的一种(抗原)Multi-class antibodies规格: 0.5mg
CASPASE3凋亡抑制剂 Anti-Z-DEVD-FMK 0.2ml
Smoothened 英文名称: 跨膜蛋白Smoothened抗体 0.1ml
Epiphycan 英文名称: 软骨素蛋白多糖3抗体 0.2ml
Rhesus antibody Rh phospho-IKK alpha (Tyr463) 化KB抑制蛋白激酶α抗体 规格 0.1ml
Trk B 神经生长因子受体的一种(抗原)Multi-class antibodies规格: 0.5mg
phospho-FAK(pSer732)(phospho-Focal adhesion kinase) 化粘着斑激酶抗原Multi-class antibodies规格: 0.5mg
细胞死亡调解子抗体 Anti-Bim 0.1ml
Rhesus antibody Rh phospho-ECT2 (Thr790) 化上皮细胞癌转化蛋白2抗体 规格 0.1ml
暗室灯 个
ZNF148 英文名称: 指蛋白148抗体 0.2ml
DAPL1 英文名称: 凋亡相关蛋白激酶样蛋白1抗体 0.2ml
细胞死亡调解子抗体 Anti-Bim 0.1ml
ZWILCH 英文名称: 着丝粒ZWILCH蛋白抗体 0.2ml
DEPDC1 英文名称: 细胞周期调控蛋白SDP35抗体 0.1ml
α-甲基酰基辅酶A消旋酶抗体 Anti-AMACR/PS 0.1ml
Anti-YBX-1/FITC 荧光素标记高保留转录因子抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-FAK(Ser722) 化粘着斑激酶抗体 规格 0.1ml
cx3cl1(chemokine (C-X3-C motif) ligand 1) 趋化因子(C-X3-C 基元)配体1抗原Multi-class antibodies规格: 0.5mg
RFPL4B contains 1 B30.2/SPRY domain and 1 RING type zinc finger. The function of RFPL4B remains unknown.
Similarity : Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
Database links : UniProtKB/Swiss-Prot: Q6ZWI9.2
Anti-rInsulin /HRP 辣根过氧化物酶标记抗重组人胰岛素抗体IgGMulti-class antibodies规格: 0.2ml
Nogo-66 1- peptide Nogo-66 (1-)多肽Multi-class antibodies规格: 0.5mg
信号转导和转录激活因子5抗体 Anti-STAT5 0.2ml
SCRO 英文名称: 鳞状细胞癌相关蛋白抗体 0.2ml
Estrogen Receptor alpha 英文名称: 雌激素受体α抗体 0.1ml
Rhesus antibody Rh Phospho-Lyn (Tyr507) 膜相关蛋白酪酸激酶Lyn抗体 规格 0.1ml
Nogo-66 1- peptide Nogo-66 (1-)多肽Multi-class antibodies规格: 0.5mg
Anti-AGT 血管紧张素原抗体Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh phospho-APS(Ser598) 化APS抗体 规格 0.1ml
Synaptophysin 突触素 浓缩液 0.1ml 进口分装
Ube2G2 英文名称: 泛素蛋白连接酶G2抗体 0.2ml
DHRS13 英文名称: 短链脱还原酶13抗体 0.2ml
Anti-AGT 血管紧张素原抗体Multi-class antibodies规格: 0.1ml
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14-3-3 protein ζ/δ抗体图片
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