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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Sodium bicarbonate
- CAS号:
144-55-8
- 保质期:
2年
- 供应商:
上海瓦兰生物
- 保存条件:
常温
- 规格:
500G
性质
| Related Categories | Apoptosis and Cell Cycle, Biological Buffers, Buffers,Chemical Synthesis, Companion Products and Reagents, 更多... |
| Quality Level | PREMIUM |
| grade | for molecular biology |
| InChI Key | UIIMBOGNXHQVGW-UHFFFAOYSA |
| product line | BioReagent |
| assay | 99.5-100.5% |
| form | powder |
| quality | Premium |
| pH range | 6.8 - 7.4 |
| pKa (25 °C) | (1) 6.37, (2) 10.25 (carbonic acid) |
| solubility | water: soluble 100 mg/mL |
| suitability | suitable for cell culture |
| suitable for insect cell culture |
产品描述
包装
1 kg in poly bottle
5 kg in poly drum
500 g in poly bottle
Application
Sodium bicarbonate has been used:
• as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]
• as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]
• as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]
• as a component of medium used for cell culture of human embryonic kidney cells[4]
• as a component of Beltsville thawing solution used for the dilution of semen[5]
General description
Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.
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文献和实验积的95%,轻微搅拌溶解。 3)加入规定量的碳酸氢钠及所要添加的物质。 4)轻微搅拌溶解,加注射用水至规定体积。 5)用1mol/L 氢氧化钠溶液或1mol/L盐酸溶液调pH至所需值。 6)用0.22μm滤膜正压过滤除菌。 7)溶液应在2℃~8℃下避光保存。 具体使用方法参照培养基包装袋上的说明书。 (2) 配制可高压灭菌细胞培养基 1)根据所需将培养基全部倒入一容器中,用少量注射用水(20℃~30℃)将袋内残留培养基洗下,并入容器。加注射用水至总体积的95%,轻微搅拌溶解
相关专题 MEM细胞培养基 GIBCO的MEM粉末包装上面有写:含谷氨酰胺,非必需氨基酸,但是不含碳酸氢钠,没有注明加碳酸氢钠的量。我是用的10%的血清的培养基,是不是配制培养基的时候用900ML的双蒸水就好了呢?需要加碳酸氢钠多少?还需要补充一些什么成分呢? 仔细看看MEM粉末的外包装标签,上面会有加碳酸氢钠的要求。如果没有,大包装盒上一定有,1L*10的大包装盒上有加碳酸氢钠的要求,1L加2.2g碳酸氢钠。否则请你的
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