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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
25 - 8000 pg/ml
- 供应商:
康朗生物
- 规格:
96T
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Popular DPP4 / CD26 Elisa Kits
Rat DPP4 / CD26 ELISA Kit (Sandwich ELISA) - LS-F22636
Reactivity: Rat
Range: 0.625-40 ng/ml
Human DPP4 / CD26 ELISA Kit (Sandwich ELISA) - LS-F1022
Reactivity: Human
Range: 32-2000 pg/ml
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Reactivity: Human
Range: 312-20000 pg/ml
Mouse DPP4 / CD26 ELISA Kit (Sandwich ELISA) - LS-F2631
Reactivity: Mouse
Range: 62.5-4000 pg/ml
Product Description
LS-F50 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human DPP4 / CD26 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of DPP4 / CD26 as low as 25 picograms per milliliter.More DPP4 / CD26 Products
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
DPP4 / CD26
Synonyms
DPP4, ADCP-2, ADABP, Dipeptidyl peptidase IV, Dipeptidyl-peptidase 4, DPPIV, ADCP2, T-cell activation antigen CD26, CD26, CD26 antigen, Dipeptidyl peptidase 4, Dipeptidylpeptidase 4, DPP IV, TP103
Reactivity
Human
Specificity
The antibody pair provided in this kit recognizes human DPP4 / CD26.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Sample Dilution
500-5000 fold
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
25 - 8000 pg/ml
Sensitivity
25 pg/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Wash Buffer Concentrate (20X)
- Standard (Lyophilized)
- Biotinylated Detection Antibody
- HRP-Streptavidin Conjugate (500x)
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B (5x)
- Adhesive Plate Sealers
Protocol
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About DPP4 / CD26
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion.P27487 NM_001935 NP_001926.2
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Sandwich ELISA Platform Overview
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
) Materials Osteocalcin sandwich ELISA kit (Biomedical Technologies, cat. no. BT‐470) including:
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