Diethyl pyrocarbonate

性质

Related Categories	Biochemicals, Carboxypeptidase P, Core Bioreagents, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme, 更多...
InChI Key	FFYPMLJYZAEMQB-UHFFFAOYSA-N
assay	≥97% (NMR)
refractive index	n20/D 1.398(lit.)
bp	93-94 °C/18 mmHg(lit.)
density	1.101 g/mL at 25 °C(lit.)
storage temp.	2-8°C

产品描述

Application

Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases.^[1] It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms.^[2]

Biochem/physiol Actions

在溶液中浓度约为 0.1% (v/v) 时可使 RNase 失活，从而防止 RNA 被降解。

DEPC is a potent nuclease inhibitor.^[3][4]0.1% solution of DEPC is used to inactive RNase. 
It inhibits the ryanodine binding to ryanodine/Ca²⁺ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca²⁺ permeability of SR vesicles.^[5]

包装

5, 25, 50, 100 mL in glass bottle

General description

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.