LS-F24481 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human DLL1 in samples of Cell Culture Supernatants, Plasma, Serum and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of DLL1 as low as 10 picograms per milliliter.
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For the detection and quantification of endogenous levels of natural and/or recombinant Human DLL1 proteins.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum, Urine
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
78 - 5000 pg/ml
Sensitivity
10 pg/ml
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated 96-well Strip Plate
Standard (Lyophilized)
Biotinylated Detection Antibody (100x)
Avidin-Biotin-HRP Complex (100x)
Sample Diluent
Biotinylated Detection Antibody Diluent
Avidin-Biotin-HRP Complex Diluent
Wash Buffer (20x)
TMB Substrate
Stop Solution
Adhesive Plate Sealers
Background
DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Restrictions
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.O00548NM_005618NP_005609.3