- ￥2835
- worthington
- 美国
- LS004125
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Collagenase, Animal Origin Free (Code: CLSAFB) which is filtered through a 0.22 micron membrane and lyophilized in vials.
- 保质期：
详见说明书
- 英文名：
Collagenase, CLSAFB, Filtered
- 库存：
大量现货供应
- 供应商：
上海经科化学科技有限公司
- CAS号：
CLSAFBS
- 规格：
5x50 mg
Collagenase, CLSAFB, Filtered
Collagenase, Animal Origin Free (Code: CLSAFB) which is filtered through a 0.22 micron membrane and lyophilized in vials.
名称：Collagenase, CLSAFB, Filtered
货号：LS004125
规格：5x50 mg
价格：2835
Code：CLSAFBS
Activity/Specification：>=300 units per mg dry weight
UNSPSC Code(s)*：12352204
Source：Clostridium histolyticum
Storage Conditions：Collagenase, Animal Origin Free (Code: CLSAFB) which is filtered through a 0.22 micron membrane and lyophilized in vials..
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文献和实验. It should be sterile filtered through a 0.22um pore size membrane. Its optimum pH range is 7-9. Calcium is required. Most balanced salt solutions have enough Ca. Collagenase is inhibited by metal chelating agents like cysteine, EDTA or o-phenanthroline. For our assay
with a sterile pipette and discard. Add the filtered working collagenase solution. Incubate for 1 to 3 hours, mixing occasionally, until the tissue is broken up. E . When the tissue in collagenase is sufficiently broken up; the solution should be cloudy
PriCells: Methods of Isolation and Culture of Human Hepatocytes
perfusion modified from the method, which was derived from the method. The major modifications were the substitution of EBSS for HEPES as the perfusion buffer and an additional washing step to remove residual EGTA prior to collagenase perfusion, adopted
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