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- abgent
- IH-0133
- 中国
- 2025年07月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
上海研卉生物科技有限公司
- 抗体英文名：
CD10 Antibody Mouse Monoclonal
- 保质期：
一年
- 规格：
6ml
Application
|
IHC |
|---|---|
| Primary Accession | P08473 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 453F9G7 |
| Gene ID | 4311 |
|---|---|
| Gene Name | MME |
| Other Names | Neprilysin, 3.4.24.11, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 24.11, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN |
| Dilution | IHC~~Ready-to-use |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Synonyms | EPN |
|---|---|
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). |
| Cellular Location | Cell membrane; Single-pass type II membrane protein |
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文献和实验上皮及间皮类常用标记抗体EPITHELIAL AND MESOTHELIAL MARKERSCK (MNF 116)- MNF-116 is a pan-cytokeratin antibody.- Source: Dakopatts.- Dilution 1:50.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically
多是多克隆；而小鼠来源的抗体多是单克隆，但也有另外。这条主要要与后面的二抗来源相匹配。（3）实验目的是检测什么种属的抗原，即species reactivity。这一点很重要，一般说明书上都有注明，如小鼠Ms、大鼠Rat、人Hum等等。（4）能否做免疫组化。一般一抗说明书都会注明做WB、IHC、ICC、IF等，建议最好选择注明的抗体，因为一般都是经过文章证明。（5）检测标本类型。用于检测石蜡切片还是冰冻切片，一般能做石蜡切片的抗体，可能都可以用来检测冰冻切片，但能做冰冻切片的抗体，不一定能检测石蜡
If titre is positive do one of the following 2 weeks later: Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR Boost subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed
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