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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见产品说明书
- 英文名:
VIAL, COVER, 30 ML (8)
- 保存条件:
见产品说明书
- 供应商:
美国
- 库存:
大量
- 规格:
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文献和实验and dip into a scintillation vial containing 5ml PBS/5ml heptane. The embryos should slide off. Those with vitelline membranes intact will stay at the interphase, while hatched larvae will settle to the bottom. Use a paint brush to remove remaining larvae
in 3% bleach until embryos float to the surface (1-5 min). Don't worry about over-dechorionating. Rinse with water. -remove screen and dip into a scintillation vial containing 5ml PBS/5ml heptane. The embryos should slide off. Those with vitelline membranes
LABELING ALPHA-ACTININ WITH IATR
Materials 1.Small vial and stir bar. 2.Bio-Beads SM-2 in 0.7x15 cm column. 3.2 mM Tris-HCl,pH 8.5,4℃,250 ml. 4.Centricon-30 concentrator. 5.5 mM Tris-acetate,0.1 mM DTT,pH 6.95,4℃,250 ml. 6.200 mM K-borate,pH 9.0,10 ml. 7.DTT,100 mM stock
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