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POROUS CELLOPHANE, 50 SHEETS
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文献和实验A Detailed Procedure for RNase Activity Staining on SDS-PAGE
of cellophane to the size of the frame -- wet the cellophane thoroughly with water -- smooth one of the two cellophane sheets over the solid backing plate -- center the gel on the cellophane -- smooth the second sheet over gel
Pour off Buffer 3, quickly place filter between 2 sheets of cellophane (or some sort of plastic sheets--we use cut-up heat seal bags), and drop 0.5 to 1.0 ml of CSPD-Buffer 3 onto filter (DNA side up). Gently spread over entire filter by pressing
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
volume withdistilled water. 6. Destaining solution: 30% (v/v) methanol and 10% (v/v) acetic acid. 7. Gel drying solution: 50% (v/v) methanol and 10% (v/v) acetic acid. 8. Cellophane wrap. 2.5. Immunoblotting 1. Mini-gel transfer apparatus to match
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