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- WB=1:200-1000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-600 IHC-F=1:100-600 IF=1:100-600
- 详见说明书
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
肿瘤抑素抗体使用说明书
- 抗体英文名：
Anti-Tumstatin/COL4A3
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:200-1000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-600 IHC-F=1:100-600 IF=1:100-600
- 宿主：
详见说明书
- 供应商：
上海博湖
- 库存：
大量
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Tumstatin C-terminus
- 规格：
0.2ml/200μg
肿瘤抑素抗体使用说明书多配体蛋白聚糖1抗体
T细胞特异性趋化因子抗体
细胞凋亡抑制因子抗体
CIDEB抗体
D型产气荚膜梭菌抗体
英文名称 Anti-Tumstatin/COL4A3
中文名称 肿瘤抑素抗体使用说明书
别 名 Collagen alpha-3(IV) chain; Alpha 3 type IV collagen; Alpha3 type IV collagen; COL4A 3; COL4A3; Collagen IV alpha 3 polypeptide; Collagen type IV alpha 3 (Goodpasture antigen); Collagen type IV alpha 3; Goodpasture antigen; OTTHUMP00000195044; Tumstatin; Col4a3; CO4A3_HUMAN.
肿瘤抑素抗体使用说明书浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 肿瘤 胶原蛋白
蛋白分子量 predicted molecular weight: 27/159kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Tumstatin C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
肿瘤抑素抗体使用说明书产品应用 WB=1:200-1000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-600 IHC-F=1:100-600 IF=1:100-600
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.
Function : Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
Subunit : here are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. The alpha 3(IV) chain forms a triple helical protomer with alpha 4(IV) and alpha 5(IV); this triple helical structure dimerizes through NC1-NC1 domain interactions such that the alpha 3(IV), alpha 4(IV) and alpha 5(IV) chains of one protomer connect with the alpha 5(IV), alpha 4(IV) and alpha 3(IV) chains of the opposite promoter, respectively. Interacts with COL4A3BP AND ITGB3. Associates with LAMB2 at the neuromuscular junction and in GBM.
Subcellular Location : Secreted, extracellular space, extracellular matrix, basement membrane. Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina(BL).
Tissue Specificity : Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.
Post-translational modifications : Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Isoform 2 contains an additional N-linked glycosylation site.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues (By similarity).
Phosphorylated by the Goodpasture antigen-binding protein/COL4A3BP.
DISEASE : Note=Autoantibodies against the NC1 domain of alpha 3(IV) are found in Goodpasture syndrome, an autoimmune disease of lung and kidney.
Defects in COL4A3 are a cause of Alport syndrome autosomal recessive (APSAR) [MIM:203780]. APSAR is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Defects in COL4A3 are a cause of benign familial hematuria (BFH) [MIM:141200]; also known as thin basement membrane nephropathy. BFH is characterized by persistent hematuria, an electron microscopically detectable thin glomerular basement membrane (GBM) and an autosomal dominant mode of inheritance. Renal function remains normal. In children, differentiation between BFH and AS can be difficult, because both disorders are manifested by persistent hematuria and thin GBM at that age.
Defects in COL4A3 are a cause of Alport syndrome autosomal dominant (APSAD) [MIM:104200]. Alport syndrome is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Similarity : Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
Database links : UniProtKB/Swiss-Prot: Q01955.3
肿瘤抑素（Tumstatin）是来源于血管基底膜Ⅳ型胶原的肿瘤血管生成抑制因子，一种自然产生的大分子多肽蛋白，特异性结合αvβ3整合素受体选择性抑制肿瘤血管内皮细胞的增殖和迁移，能够抑制原始血管外生长出新的血管，从而抑制肿瘤生长、增值，肿瘤抑素具有很强的生物学效应。
肿瘤抑素抗体使用说明书抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体 抗体的交叉反应： 人，小鼠，大鼠，鸡 ，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。
指蛋白855抗体 Anti-ZBTB48 WB IHC-P IHC-F IF 100ul
指蛋白ZBTB5抗体 Anti-ZBTB5 WB IHC-P IHC-F IF 100ul
指蛋白ZBTB6抗体 Anti-ZBTB6 WB IHC-P IHC-F IF 100ul
指蛋白ZBTB7C抗体 Anti-ZBTB7C WB IHC-P IHC-F IF 100ul
指蛋白ZBTB8A抗体 Anti-ZBTB8A WB IHC-P IHC-F IF 100ul
凋亡加强结构域蛋白14抗体 Anti-CARD14 WB IHC-P IHC-F IF 100ul
肌酸激酶M型抗体 Anti-Creatine Kinase MM WB IHC-P IHC-F IF 100ul
NK细胞受体BY55抗体 Anti-CD160/By55 WB IHC-P IHC-F IF 100ul
凝集胎盘蛋白1抗体 Anti-COLEC12 WB IHC-P IHC-F IF 100ul
活化诱导分子CD69抗体 Anti-CD69 WB IHC-P IHC-F IF 100ul
金氏B培养基(KBA)250g用于检测假单胞菌的荧光素
金氏B培养基 King’s B medium 用于铜绿假单菌产荧光色素试验
乳酸菌选择性培养基 Raka-Ray Medium 250克 啤酒中乳酸菌检测和分离培养
标准II号营养琼脂250细菌计数、不含糖，可作血琼脂基础和传代用(MERCK方法)incubationmedia标准II号营养琼脂250细菌计数、不含糖，可作血琼脂基础和传代用(MERCK方法)
胆乳琼脂(DHL) 250g 肠道菌选择性培养基，特别是沙门氏菌的选择性分离(GB、SN标准)
甘露醇録琼脂培养基220250g用于金黄色葡萄球菌的选择性分离培养和计数(《中华人民共和国药典》20。
LB Broth Agar 培养基 250g incubation media LB Broth Agar 培养基 250g
亚黄八叠球菌 支/瓶
theSmallestBrothMedium
碱性L-G培养基基础 250g 用于酸性物质处理的结核杆菌标本
肿瘤抑素抗体使用说明书乙酰胺琼脂250g用于绿脓杆菌的选择性分离培养(GB标准)
假单胞分离肉汤 250(g) incubation media 假单胞分离肉汤 250(g)
脱氧核糖核酸酶甲基绿琼脂基础 Dnase Agar Base With Methyl Green 100克 用于细菌的DNA酶水解试验
録化孔雀绿肉汤(MM，RVMedium)250用于沙门氏菌的选择性增菌培养(GB标准)incubationmedia録化孔雀绿肉汤(MM，RVMedium)250用于沙门氏菌的选择性增菌培养(GB标准)
IndoleMe
肿瘤抑素抗体使用说明书实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
T细胞特异性趋化因子抗体
细胞凋亡抑制因子抗体
CIDEB抗体
D型产气荚膜梭菌抗体
英文名称 Anti-Tumstatin/COL4A3
中文名称 肿瘤抑素抗体使用说明书
别 名 Collagen alpha-3(IV) chain; Alpha 3 type IV collagen; Alpha3 type IV collagen; COL4A 3; COL4A3; Collagen IV alpha 3 polypeptide; Collagen type IV alpha 3 (Goodpasture antigen); Collagen type IV alpha 3; Goodpasture antigen; OTTHUMP00000195044; Tumstatin; Col4a3; CO4A3_HUMAN.
肿瘤抑素抗体使用说明书浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat
产品类型 一抗
研究领域 肿瘤 胶原蛋白
蛋白分子量 predicted molecular weight: 27/159kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Tumstatin C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
肿瘤抑素抗体使用说明书产品应用 WB=1:200-1000 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-600 IHC-F=1:100-600 IF=1:100-600
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.
Function : Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.
Subunit : here are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. The alpha 3(IV) chain forms a triple helical protomer with alpha 4(IV) and alpha 5(IV); this triple helical structure dimerizes through NC1-NC1 domain interactions such that the alpha 3(IV), alpha 4(IV) and alpha 5(IV) chains of one protomer connect with the alpha 5(IV), alpha 4(IV) and alpha 3(IV) chains of the opposite promoter, respectively. Interacts with COL4A3BP AND ITGB3. Associates with LAMB2 at the neuromuscular junction and in GBM.
Subcellular Location : Secreted, extracellular space, extracellular matrix, basement membrane. Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina(BL).
Tissue Specificity : Alpha 3 and alpha 4 type IV collagens are colocalized and present in kidney, eye, basement membranes of lens capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal kidney and fetal lung. PubMed:8083201 reports similar levels of expression of alpha 3 and alpha 4 type IV collagens in kidney, but PubMed:7523402 reports that in kidney levels of alpha 3 type IV collagen are significantly lower than those of alpha 4 type IV collagen. According to PubMed:8083201, alpha 3 type IV collagen is not detected in heart, brain, placenta, liver, pancreas, extrasynaptic muscle fibers, endoneurial and perineurial nerves, fetal brain, fetal heart and fetal liver. According to PubMed:7523402, alpha 3 type IV collagen is strongly expressed in pancreas, neuroretina and calvaria and not expressed in adrenal, ileum and skin. Isoform 1 and isoform 3 are strongly expressed in kidney, lung, suprarenal capsule, muscle and spleen, in each of these tissues isoform 1 is more abundant than isoform 3. Isoform 1 and isoform 3 are expressed at low levels in artery, fat, pericardium and peripherical nerve, but not in placenta, mesangium, skin, pleura and cultured umbilical endothelial cells.
Post-translational modifications : Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Isoform 2 contains an additional N-linked glycosylation site.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues (By similarity).
Phosphorylated by the Goodpasture antigen-binding protein/COL4A3BP.
DISEASE : Note=Autoantibodies against the NC1 domain of alpha 3(IV) are found in Goodpasture syndrome, an autoimmune disease of lung and kidney.
Defects in COL4A3 are a cause of Alport syndrome autosomal recessive (APSAR) [MIM:203780]. APSAR is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Defects in COL4A3 are a cause of benign familial hematuria (BFH) [MIM:141200]; also known as thin basement membrane nephropathy. BFH is characterized by persistent hematuria, an electron microscopically detectable thin glomerular basement membrane (GBM) and an autosomal dominant mode of inheritance. Renal function remains normal. In children, differentiation between BFH and AS can be difficult, because both disorders are manifested by persistent hematuria and thin GBM at that age.
Defects in COL4A3 are a cause of Alport syndrome autosomal dominant (APSAD) [MIM:104200]. Alport syndrome is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness.
Similarity : Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
Database links : UniProtKB/Swiss-Prot: Q01955.3
肿瘤抑素（Tumstatin）是来源于血管基底膜Ⅳ型胶原的肿瘤血管生成抑制因子，一种自然产生的大分子多肽蛋白，特异性结合αvβ3整合素受体选择性抑制肿瘤血管内皮细胞的增殖和迁移，能够抑制原始血管外生长出新的血管，从而抑制肿瘤生长、增值，肿瘤抑素具有很强的生物学效应。
肿瘤抑素抗体使用说明书抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体 抗体的交叉反应： 人，小鼠，大鼠，鸡 ，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。
指蛋白855抗体 Anti-ZBTB48 WB IHC-P IHC-F IF 100ul
指蛋白ZBTB5抗体 Anti-ZBTB5 WB IHC-P IHC-F IF 100ul
指蛋白ZBTB6抗体 Anti-ZBTB6 WB IHC-P IHC-F IF 100ul
指蛋白ZBTB7C抗体 Anti-ZBTB7C WB IHC-P IHC-F IF 100ul
指蛋白ZBTB8A抗体 Anti-ZBTB8A WB IHC-P IHC-F IF 100ul
凋亡加强结构域蛋白14抗体 Anti-CARD14 WB IHC-P IHC-F IF 100ul
肌酸激酶M型抗体 Anti-Creatine Kinase MM WB IHC-P IHC-F IF 100ul
NK细胞受体BY55抗体 Anti-CD160/By55 WB IHC-P IHC-F IF 100ul
凝集胎盘蛋白1抗体 Anti-COLEC12 WB IHC-P IHC-F IF 100ul
活化诱导分子CD69抗体 Anti-CD69 WB IHC-P IHC-F IF 100ul
金氏B培养基(KBA)250g用于检测假单胞菌的荧光素
金氏B培养基 King’s B medium 用于铜绿假单菌产荧光色素试验
乳酸菌选择性培养基 Raka-Ray Medium 250克 啤酒中乳酸菌检测和分离培养
标准II号营养琼脂250细菌计数、不含糖，可作血琼脂基础和传代用(MERCK方法)incubationmedia标准II号营养琼脂250细菌计数、不含糖，可作血琼脂基础和传代用(MERCK方法)
胆乳琼脂(DHL) 250g 肠道菌选择性培养基，特别是沙门氏菌的选择性分离(GB、SN标准)
甘露醇録琼脂培养基220250g用于金黄色葡萄球菌的选择性分离培养和计数(《中华人民共和国药典》20。
LB Broth Agar 培养基 250g incubation media LB Broth Agar 培养基 250g
亚黄八叠球菌 支/瓶
theSmallestBrothMedium
碱性L-G培养基基础 250g 用于酸性物质处理的结核杆菌标本
肿瘤抑素抗体使用说明书乙酰胺琼脂250g用于绿脓杆菌的选择性分离培养(GB标准)
假单胞分离肉汤 250(g) incubation media 假单胞分离肉汤 250(g)
脱氧核糖核酸酶甲基绿琼脂基础 Dnase Agar Base With Methyl Green 100克 用于细菌的DNA酶水解试验
録化孔雀绿肉汤(MM，RVMedium)250用于沙门氏菌的选择性增菌培养(GB标准)incubationmedia録化孔雀绿肉汤(MM，RVMedium)250用于沙门氏菌的选择性增菌培养(GB标准)
IndoleMe
肿瘤抑素抗体使用说明书实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
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文献和实验相关实验
性染色，使阴性细胞的背景荧光降至未标记细胞的水平。加入0.5-1μg纯的抗小鼠CD16/32单克隆抗体，室温孵育10分钟。 对于人和大鼠，可直接使用过量的与荧光抗体相同来源和亚型的纯化Ig或者相同来源血清进行阻断，或者用商业化的Fc受体阻断剂。 细胞染色 4.1 按照说明书的推荐用量加入荧光标记的抗体，混匀后置于4℃，避光孵育30分钟。 4.2 加入适量细胞染色buffer (或含1%BSA的PBS)重悬细胞，300g离心细胞悬液5分钟，弃掉上清。 4.3 加入0.2 mL
抗体保存得当与否，直接决定了抗体的活性和使用效果！如果抗体保存得当，大部分抗体活性都可以维 持数月甚至数年。 本指南以 abcam 抗体保存方法为主，同样也可以应用到其他绝大部分公司的抗体产品！ 请谨记！无论何时 请按照说明书推荐的保存条件正确保存抗体！ 1、 收到抗体后请务必在 12000rpm 离心 1-5分钟后再打开管盖进行分装和保存！ Abcam 的抗体使用非常特殊的储存管，只有在12000rpm离心1-5分钟才能将附着在管壁或盖内的抗体全部 离心下来。即使是在10000
特异性的选择主要需要考虑四个方面：蛋白特异性、种属特异性、实验方法特异性、标记物的特异性。 （1）蛋白特异性 针对需要检测的蛋白查找抗体，几个细节要区分，重组表达的蛋白和内源性蛋白的检测，对抗体的要求是不一样的，注意查看抗体说明书的检测说明。如果重组蛋白不是全长表达，则需要注意抗体的免疫原区域是否在重组蛋白区域内。内源性蛋白最好能清楚其剪切与修饰的方式，特殊表型的蛋白需要进行序列比对，并结合抗体免疫原序列，查看交叉反应的情况。磷酸化蛋白检测需要确定具体位点，不同位点的磷酸化意味
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肿瘤抑素抗体使用说明书
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