established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described in the literature to contain parathyroid-like activity
Species:
human (Homo sapiens)
Tissue:
thyroid
Disease:
thyroid carcinoma
Gender:
N/A
Morphology:
epithelial-like cells growing as monolayer
Growth Mode:
N/A
Doubling Time:
ca. 100 hours
DNA Profile:
N/A Cell Line Authentication Service
Culture Medium:
DMEM+20%FBS We strongly suggest to purchase the complete medium
Cryopreservation medium:
90%FBS+10%DMSO
Mycoplasma:
contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics and species PCR
Cytogenetics:
human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc.