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文献和实验Isolation of RNA from Difficult Tissues
ToTALLY RNA™ Kit). Note that many of these techniques can be used with other RNA isolation protocols as well. Heart and Skeletal Muscle: Fibrous Tissues For fibrous tissues such as rat and mouse heart and skeletal muscle, the most difficult step
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
The immunocytochemical procedure described represents an original modification of the method for cell death detection by ELISA. Utilizing this new modified assay we are able to show the presence of apoptotic cells or cells involved in apoptosis in the thymus
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Support Protocol 3: Mouse Euthanasia and Tissue Dissection Support Protocol 4: Serum Insulin Quantification Using ELISA Support Protocol 5: Analysis of Human Cell Engraftment by Flow Cytometry
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