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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Transgenic Mouse and Transgenic Rat Outline
with any questions or Meet the Staff. 2. Plan the experiment. What is the purpose of your experiment? Do you want to define tissue specific regulatory sequences? Do you want to overexpress a protein in a specific cell lineage? You will need to obtain or clone
Isolation of Protein from Tissue
or Fibroblasts Collect cells (lymphoblast or fibroblast)from tissue culture flask and wash 3 times with 1X phosphate buffer saline (PBS),pH 7.4.I add 100µl Brij buffer (for cells collected from 75mm flask).Sonicate the cell pellets for 45 seconds
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