Human Complement C8b ELISA Kit

Human Complement C8b ELISA Kit (Sandwich ELISA) - LS-F50793

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LS-F50793-1
1 plate
$1,090 USD
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Product Description

LS-F50793 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Complement C8b in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Complement C8b as low as 1.37 picograms per milliliter.
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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Complement C8b
Synonyms
 
C8B, Complement C8B
Reactivity
 
Human
Specificity
 
This assay has high sensitivity and excellent specificity for detection of human C8B. No significant cross-reactivity or interference between human C8B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human C8B and all the analogues, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Plasma, Serum, Tissue Homogenates
Sample Size
 
50 - 100 µl
Format
 
96-Well Strip Plate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
2.74 - 2000 pg/ml
Sensitivity
 
1.37 pg/ml
Assay Time
 
2.5 h
Precision
 
Intra-Assay: CV<15% / Inter-Assay: CV<15%
Storage
 
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Adhesive Plate Sealers
• Sample Diluent
• Positive Control
• Negative Control'
• TMB Substrate
• Standard (Lyophilized)
• HRP Conjugate Diluent
• HRP Conjugate (100x)

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About Complement C8b
 
Complement C8b is one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor.Uniprot: P07358 NCBI: NM_000066 NP_000057.2

 

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