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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
21 - 5000 ng/ml
- 供应商:
康朗生物
- 规格:
96T
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Popular Complement C4c Elisa Kits
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Product Description
LS-F49866 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Complement C4c in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Complement C4c as low as 21 nanograms per millilter.More Complement C4c Products
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Complement C4c
Reactivity
Human
Specificity
Human C4c
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
21 - 5000 ng/ml
Sensitivity
21 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12%
Storage
Store at 4°C for 6 months or at -20°C for 1 year. Avoid freeze/thaw cycles.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Adhesive Plate Sealers
- Assay Diluent A
- Assay Diluent B
- Assay Diluent C
- Wash Buffer (20x)
- TMB Substrate
- HRP-Streptavidin Conjugate (200x)
- Stop Solution
- Standard (Lyophilized)
- Biotinylated Detection Antibody
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About Complement C4c
Complement component 4 is a protein involved in the complement system. It is cleaved into proteins 4a and 4b; 4b can be further cleaved into 4c and 4d. C4a is an anaphylatoxin. C4b forms part of C3-convertase, in conjunction with 2a, and can bind CR1. C4d has been identified as a biomarker for systemic lupus erythematosus.
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Sandwich ELISA Platform Overview
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Evaluation of total complement function in human serum is an essential component of laboratory diagnostics of the human complement system. During recent years, established hemolytic assays for classical pathway and alternative pathway
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Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Human complement component C2 is a critical factor of the classical complement pathway. Here we provide a method for the production of recombinant human C2 (rhC2) protein for research purposes. The human complement component C2 (hC
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