Human Complement C4b ELISA Kit

Human Complement C4b ELISA Kit (Sandwich ELISA) - LS-F22486

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LS-F22486-1
1 plate
$740 USD
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Product Description

LS-F22486 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Complement C4b in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Complement C4b as low as 4.688 nanograms per millilter.
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Specifications

Type
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Complement C4b
Synonyms
 
C4B, C4B12, C4BD, C4B1, C4B3, Complement C4B, CH, Chido form of C4, CPAMD3, Complement C4-B, CO4, Basic complement C4, C4B2, C4B5, C4F, Complement C4B1a, Complement component 4B
Reactivity
 
Human
Specificity
 
This kit recognizes natural and recombinant Human C4b.
Manual
 

Intended Sample Types
 
Plasma, Serum
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
4.688 - 300 ng/ml
Sensitivity
 
4.688 ng/ml
Assay Time
 
3.5 h
Precision
 
Intra-Assay: CV<5.40% Inter-Assay: CV<6.23%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated 96-well Strip Plate
• Standard (Lyophilized)
• Sample Diluent
• Biotinylated Detection Antibody (100x)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate (100x)
• HRP Conjugate Diluent
• Wash Buffer (25x)
• TMB Substrate
• Stop Solution
• Adhesive Plate Sealers

Protocol
 

Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
About Complement C4b
 
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. NCBI: U24578 AAA99717.1

 

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